|本期目录/Table of Contents|

[1]杨清林△,蔡绍皙.磷脂双层膜(sBLM)对 ss-DNA的电化学响应研究[J].生物医学工程研究,2011,04:248-250.
　YANG Qinglin,Cai Shaoxi.Research on the Electrochemical Response of sBLM to ssDNA[J].Journal of Biomedical Engineering Research,2011,04:248-250.
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磷脂双层膜(sBLM)对 ss-DNA的电化学响应研究(PDF)

《生物医学工程研究》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2011年04期
页码:
248-250
栏目:
论著
出版日期:
2011-12-30

文章信息/Info

Title:
Research on the Electrochemical Response of sBLM to ssDNA
作者:
杨清林1△; 蔡绍皙2
1.重庆科技学院化学化工学院, 重庆 401331；2.重庆大学生物工程学院，重庆 400030
Author(s):
YANG Qinglin1 ; Cai Shaoxi2
1 Chemistry and Chemical of Engineering College，Chongqing University of Science & Technology , Chongqing 401331,China; 2 College of Bioengineering of Chongqing University, Chongqing 400030
关键词:
磷脂双层膜; s-BLM; 循环伏安法; ss-DNA; 生物传感器
Keywords:
Bilayer lipid membranes;  s-BLM ; Cyclic Voltammetry;  ss-DNA;  Biosensor
分类号:
R318
DOI:
-
文献标识码:
A
摘要:
利用循环伏安法，以铂电极支撑的磷脂双层膜(s-BLM) 作为生物膜的模型, 研究了连接有芘（pyrene）和四氯荧光素（TET）的Oligo d(T20)对磷脂双层膜电化学行为的影响，结果显示s-BLM 与Oligo d(T20)之间可以发生比较强烈的相互作用, 导致了膜电压和膜结构发生了差异性改变，这种现象的出现可能是由于连接有芘和四氯荧光素的Oligo d(T20)与磷脂双层膜发生作用时引起s-BLM 表面分子的排列变化, 改变了膜的通透性，这为构建整合有ss-DNA磷脂双层膜生物传感器装置提供可能。
Abstract:
By Cyclic Voltammetry，the responses of s-BLM to ss-DNA linked with tetrachloro-fluorescein(TET) and pyrene were investigated. The response of s-BLM to ss-DNA turned up strongly each other,result show that the voltage and configuration of BLM is changed differently.It is indicated that the permeability of BLM is changed , the biosensor system based on s-BLM integrated with ssDNA is likely to establish.

参考文献/References

［1］M.Kerker(ed.).Surface chemistry and colloids,Vol.7.MPT International Review of (A.D.Buc-kingham,Consultant Ed.)［M］.Butterworths,London.1972.
［2］Mueller P,Rudin D O,Tien H T,et al.Reconstitution of cell membrane structure in vitro and its transformation into an excitable system［J］.Nature,1962，194：979.
［3］Tien HT,Salamon Z.Formation of self-assembled lipid bilayers on solid substrates［J］.Bioelectrochemistry and Bioenergetics,1989，22:211-218.
［4］毕只初.微型支撑双层类脂膜pH电极的研制［J］.膜科学与技术,2002，22（5）：25-31.
［5］Dimitrios P.Nikolelis,Syrago-Styliani E.A minisensor for the rapid screening of atenolol in pharma ceu-tical preparations based on surface-stabilized bilayer lipid membranes with incorporated DNA［J］.Bioelectrochemistry，2002,58：107–112.
［6］蔡红,张占军,王斌，等.S-BLM修饰电极对组胺电化学传感行为的研究［J］.化学物理学报，2004，17（2）：206-210.

备注/Memo

备注/Memo:
收稿日期：2011-09-01 △通信作者Email:qlyangcqu@yahoo.com.cn
更新日期/Last Update: 2011-12-30