|本期目录/Table of Contents|

[1]顾伟,罗旋,匡勇△.脐带间充质干细胞辅助游离脂肪的移植研究[J].生物医学工程研究,2015,02:128-131.
 GU Wei,LUO Xuan,KUANG Yong.Research of Umbilical Cord Mesenchymal Stem Cells Assisted Adipose Tissue Transplantation[J].Journal of Biomedical Engineering Research,2015,02:128-131.
点击复制

脐带间充质干细胞辅助游离脂肪的移植研究(PDF)

《生物医学工程研究》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2015年02期
页码:
128-131
栏目:
出版日期:
2015-06-25

文章信息/Info

Title:
Research of Umbilical Cord Mesenchymal Stem Cells Assisted Adipose Tissue Transplantation
文章编号:
1672-6278 (2015)02-0128-04
作者:
顾伟1罗旋2匡勇1△
1.上海中医药大学附属曙光医院,上海 200021;2.上海交通大学医学院附属仁济医院,上海 200001
Author(s):
GU Wei1LUO Xuan2KUANG Yong1
1.Shu guang Hospital,Shanghai Traditional Medcine University,Shanghai 200021,China;2.Renji Hospital,Shanghai Jiao Tong University,Shanghai 200001
关键词:
游离脂肪移植脐带间充质干细胞细胞辅助的脂肪移植血管内皮细胞生长因子聚合酶链式反应
Keywords:
Adipose tissue transplantationUmbilical cord mesenchymal stem cellsCell assisted lipotransferVascular endothelial growth factorPolymerase chain reaction
分类号:
R318
DOI:
-
文献标识码:
A
摘要:
研究脐带间充质干细胞是否能够有效辅助游离脂肪移植。 应用脐带间充质干细胞与自体脂肪组织颗粒混合后,移植到裸鼠皮下,与单纯脂肪组织移植作对照,不同的时间观察其各组移植重量,取下后立即固定,包埋、切片,HE染色,观察组织内细胞成活及血管生成情况,通过realtime PCR技术进行检测移植物中的VEGF、PPAR-γ基因表达水平。脐带间充质干细胞可以提高移植物重量及VEGF基因表达水平,但在术后一周、一月、两月观测点与对照组相比差异均没有统计学意义。脐带间充质干细胞不能有效提高移植物重量、成脂以及血管生成的修复效应,不能有效减少术后移植脂肪的吸收,目前应用于游离脂肪移植尚不成熟。
Abstract:
To study the effect of supplement umbilical cord mesenchymal cells (UCMSCs) on adipose tissue transplantation. We used supplement UCMSCs to fat transplantation, then harvested the transplantation tissue at 1week, 1 month, 2 months after surgery, the weight of the drafted tissue was measured.HE staining was used to analysis the absorption,cysts, fibrosis within the tissue, real-time PCR was performed to detect PPAR-γ,VEGF gene expression. UCMSCs could help to increase the graft tissue weigtht and VEGF to a certain degree, though there was no statistical difference at 1week, 1 month and.2 months post-transplantation. UCMSCs can not to increase vascularization and adipogenesis or decrease the absorption of the graft fat significantly, it’s still doubtful to apply UCMSCs in adipose tissue transplantation.

参考文献/References

备注/Memo

备注/Memo:
(收稿日期:2015-02-05)通信作者Email:yongk58@hotmail.com
更新日期/Last Update: 2016-08-09