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Title:: Establishment and analysis of finite element model of cell damage based on atomic force microscope

作者:: 杜美洁1; 王立2; 王彬1; 3△; 李志强1; 闫晓鹏1; 1.太原理工大学机械与运载工程学院，太原 030024;2.太原理工大学生物医学工程学院，太原 030024;3.College of Engineering, Design and Physical Sciences, Brunel University London, UK

Author(s):: DU Meijie1; WANG Li2; WANG Bin1; 3; LI Zhiqiang1; YAN Xiaopeng1; 1.College of Mechanical and Vehicle Engineering of Taiyuan University of Technology, Taiyuan 030024, China;2.School of Biomedical engineering, Taiyuan 030024;3.College of Engineering, Design and Physical Sciences, Brunel University London, UK

Keywords:: Hela cells; Atomic force microscope; Cell finite element model; Biomechanics; Breakthrough force

摘要:: 为获得细胞膜受冲击破损的实际应力应变值。应用原子力显微镜(atomic force microscope, AFM)对Hela细胞进行破膜实验，得到细胞几何参数和加载过程的力-位移曲线。基于实验所得表面形貌与材料参数建立细胞破损有限元模型，并使用ABAQUS进行分析。当针尖位移达到2.35 μm时，实验所得破膜力为38.7 nN，模拟所得破膜力为39.3 nN，两者较为吻合，验证了模型的合理性和正确性。通过模拟获得细胞膜被穿破时，应力为24.3 kPa，塑性应变为0.23。本研究基于实验建立的细胞破损有限元模型，为模拟细胞的失效与破损过程提供了方法和参考。

Abstract:: To obtain the strength characters of the cell membrane damaged by impact loading. A combined approach of experiment and numerical simulation was adopted. Hela cells were subjected to membrane penetration experiment by atomic force microscopy, and the cell geometry parameters and the force-displacement curves of the loading process were obtained. The finite element model of cell damage was established based on the experimental surface morphology and material parameters, and ABAQUS/Explicit was used for simulation.The results showed that when the AFM tip displacement reached 2.35 μm, the breakthrough force value was 38.7 nN in the experiment and 39.3 nN in the simulation, showing good agreement. From the simulation, the stress in the cell membrane was 24.3 kpa and the plastic strain was 0.23 at the punctured location. It is concluded that the finite element model of cell damage established in this paper provides a method and reference for simulating the process of cell failure and damage.