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[1]周俊涛,潘艳,周超,等.基于层层自组装技术与季铵盐类抗菌剂接枝制备表面抗菌涂层的研究*[J].生物医学工程研究,2019,03:347-352.
 ZHOU Juntao,PAN Yan,ZHOU Chao,et al.Preparation of surface antibacterial coating based on layer-by-layer self-assembly technology and grafting of quaternary ammonium salt antibacterial agent[J].Journal of Biomedical Engineering Research,2019,03:347-352.
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基于层层自组装技术与季铵盐类抗菌剂接枝制备表面抗菌涂层的研究*(PDF)

《生物医学工程研究》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2019年03期
页码:
347-352
栏目:
出版日期:
2019-09-25

文章信息/Info

Title:
Preparation of surface antibacterial coating based on layer-by-layer self-assembly technology and grafting of quaternary ammonium salt antibacterial agent
文章编号:
1672-6278 (2019)03-0347-06
作者:
周俊涛12潘艳2周超2邓林红2△
1. 常州大学材料科学与工程学院,江苏常州 213164;2. 常州大学生物医学工程与健康科学研究院,江苏常州 213164
Author(s):
ZHOU Juntao12PAN Yan2ZHOU Chao2DENG Linhong2
1. School of Materials Science & Engineering,Changzhou University,Changzhou 213164,China;2. Institute of Biomedical Engineering and Health Sciences,Changzhou University,Changzhou 213164
关键词:
层层自组装抗细菌粘附抗细菌生物膜点击反应细胞毒性
Keywords:
Layer-by-layer self-assembly Anti-bacterial adhesion Anti-biofilm Click reaction Cytotoxicity
分类号:
R318;TB34
DOI:
10.19529/j.cnki.1672-6278.2019.03.17
文献标识码:
A
摘要:
通过层层自组装技术制备的抗菌涂层一般具有抗细菌粘附的能力,但不具备直接杀灭细菌的功能,故抗菌性能不佳。本研究通过层层自组装技术与“巯基-烯”点击反应相结合,将末端为巯基修饰的季铵盐类抗菌剂接枝到自组装涂层的含氧降冰片烯结构中,赋予涂层表面杀菌的性能。通过红外光谱(FT-IR)、核磁共振(1HNMR)、石英晶体微天平(QCM)以及水接触角测试,分别对表面进行结构及物理性能表征;并初步探讨了抗菌涂层的生物毒性,以及对金黄色葡萄球菌和大肠杆菌的抗菌性能。测试结果表明,本研究获得的涂层生物相容性较好;未接枝抗菌剂的自组装涂层具有表面抗细菌粘附的能力,在接枝了抗菌剂后,涂层不但可以抗细菌粘附,而且可以杀灭表面的细菌,有效抑制细菌生物膜的形成,可望应用于生物医用材料表面高抗菌性的功能化处理。
Abstract:
Antibacterial coatings prepared by layer-by-layer selfassembly technique (LBL) can repel bacteria adhesion, but not kill bacterial directly, which limits the application in practice. Here, we reported an antibacterial coating that combined the thiol-ene click reaction with LBL to graft the thiol-modified antibacterial agent onto the coating with oxygen-containing norbornene structure , which enhanced the coating’s antibacterial property. The surface and physical properties of the coating were characterized by FT-IR, 1HNMR, QCM and water contact angle test. The biotoxicity and the antibacterial properties against? S.aureus? and? E.coli? of the antibacterial coating were evaluated. The results show that the modified coating has excellent biocompatibility. The coating without antibacterial agent has the ability to antibacterial adhesion. Furthermore, after grafting the antibacterial agent, the coating can not only resist bacterial adhesion, but also kill bacteria on the surface and effectively inhibit the formation of bacterial biofilm. Thus, the coating may have great potential to be used for the surface functionalization of biomedical materials to enhance the antibacterial properties.

参考文献/References

备注/Memo

备注/Memo:
(收稿日期:2019-03-09)国家自然科学基金资助项目(31670950,11532003);江苏省自然科学基金资助项目(BK20180963)。△通信作者Email:dlh@cczu.edu.cn〖FQ)〗
更新日期/Last Update: 2019-10-24