文章摘要

文章信息/Info

Title:: Methodological verification of characterization of traditional Chinese medicine based on delayed luminescence of scenedesmus

作者:: 闵令圆1; 2; 庞靖祥2; 杨美娜2; 周宝宸2; 荆绮2; 韩金祥2△; 1. 济南大学山东省医学科学院医学与生命科学学院，济南 250020；2. 山东省医药生物技术研究中心山东第一医科大学(山东省医学科学院)，济南 250062

Author(s):: MIN Lingyuan1; 2; PANG Jingxiang2; YANG Meina2; ZHOU Baochen2; JING Qi2; HAN Jinxiang2; 1.School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250020, China;2.Shandong Medicinal Biotechnology Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250062

Keywords:: Scenedesmus; Method validation; Biophotonics; Biological indicators; Traditional Chinese medicine; Delayed luminescence

摘要:: 对栅藻延迟发光定量化表征中药寒热药性的方法进行优化，并进行方法学验证。用YPMS-2生物光子测量仪检测栅藻激发延迟发光强度，对测得的激发延迟发光强度用Statistica 10.0软件进行线性拟合，得到表征栅藻性质的可靠参数K,用产自于道地产地的经典寒性中药黄芩作为样本，验证本研究方法的精密度、稳定性、重复性，并进行空白实验。栅藻最佳使用K值波动范围是2.81~3.89，本研究方法精密度高，重复性、稳定性良好，空白溶剂无干扰。

Abstract:: The method of quantitatively characterizing the cold and hot medicinal properties of Chinese medicinal herbs was optimized for delayed luminescence, and methodological verification was carried out. The YPMS-2 biophoton measuring instrument was used to detect the delayed luminescence intensity of the S. cerevisiae. The measured excitation delay luminescence intensity was linearly fitted with Statistica 10.0 software to obtain a reliable parameter K for characterizing the properties of S. cerevisiae. The classical cold Chinese medicine Astragalus membranaceus was used as a sample to study the precision, stability, and repeatability of the method and to carry out blank experiments. The optimum K-value fluctuation range of Scenedesmus is 2.81~3.89. The method has high precision, good repeatability and stability, and no interference from blank solvent.