|本期目录/Table of Contents|

[1]李颖,Bing Song,殷涛,等.低频变化电场促进细胞定向移动的研究*[J].生物医学工程研究,2021,02:105-113.
 LI Ying,Bing Song,YIN Tao,et al.Study on enhanced directional movements of the cells by low-frequency varying electric fields[J].Journal of Biomedical Engineering Research,2021,02:105-113.
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低频变化电场促进细胞定向移动的研究*(PDF)

《生物医学工程研究》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2021年02期
页码:
105-113
栏目:
出版日期:
2021-06-25

文章信息/Info

Title:
Study on enhanced directional movements of the cells by low-frequency varying electric fields
文章编号:
1672-6278 (2021)02-0105-09
作者:
李颖1Bing Song2殷涛1刘志朋1
1.中国医学科学院北京协和医学院生物医学工程研究所,天津 300192;2.中国科学院深圳先进技术研究院生物医学与健康工程研究所转化医学研究与发展中心,深圳 518055
Author(s):
LI Ying1Bing Song2YIN Tao1LIU Zhipeng1
1.Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China;2.Center for Translational Medicine Research and Development,Institute of Biomedical and Health Engineering,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China
关键词:
盘基网柄菌细胞迁移细胞趋向方向运动单向变化电场频率变化
Keywords:
Dictyostelium discoideumCell migrationCells′ taxisDirectional movementUnipolar varying electric fieldFrequency change
分类号:
R318;Q274;R49
DOI:
10.19529/j.cnki.1672-6278.2021.02.01
文献标识码:
A
摘要:
细胞趋电性在伤口愈合、胚胎形成、神经再生等领域至关重要。前期研究表明,细胞在脉冲电场下也能够定向移动,但目前多种非直流电场也被临床证实能有效地促进伤口愈合,而其机制尚不清楚。本研究设计了能产生连续变化电场的电刺激器,研究分析低频(10、5、1 Hz)正弦波、三角波和斜坡波电场下阿米巴细胞的运动特性,发现在正弦波电场组中,5 Hz电场下细胞比1 Hz导向性更高(P<0.01);在三角波电场组中,5 Hz电场下细胞迁移速率最高(P<0.001,P<0.001);在斜坡波电场组中,10 Hz电场下细胞导向性比5 Hz和1 Hz均低(P<0.001,P<0.001)而5 Hz电场下细胞迁移速率高于1 Hz(P<0.05),综合考虑5 Hz趋电性最好。把上述三组中最优电场与直流电场和脉冲电场对比,发现上述三种电场下细胞运动特性均优于50%占空比脉冲电场,更重要的是,5 Hz斜坡波下细胞导向性与直流电场下无明显差异(ns),而迁移速率优于后者(P<0.001),是本研究中具有最佳趋电性的电场。实验结果表明,低频变化电场也能够引起细胞定向迁移,不同波形电场下细胞趋电性差异明显,而5 Hz斜坡波电场具有基础和临床研究的应用潜力。
Abstract:
Electrotaxis, or galvanotaxis, is essential in wound healing, embryogenesis, and nerve regeneration. Previous studies have shown that pulsed electric fields can also cause directional movements of the cells as that under direct current (DC) electric fields (EFs). However, clinical studies have shown that many non-DC EFs can also effectively promote wound healing, albeit the underlying mechanism remains elusive. We designed an electric stimulator which could generate continuously varying EFs to study and analyze the motion characteristics of amoeba cells under low-frequency (10, 5 and 1 Hz) EFs with sinusoidal, triangular and ramp waveforms. It was found that in the sinusoidal group, cells under 5 Hz EF had higher directedness than those under 1 Hz (P<0.01); In the triangular group, the cell migration rate under 5 Hz EF was the highest (P<0.001,P< 0.001); In the ramp group, the cell directedness under 10 Hz EF was lower than that under 5 Hz and 1 Hz (P<0.001, P<0.001), and the migration rate under 5 Hz EF was higher than that at 1 Hz (P<0.05), therefore 5 Hz EF was the best. Compared these three optimal EFs with the DC EF and pulsed EF, it was found that the movement characteristics of cells under those three EFs were better than that under the 50% duty cycle pulsed EF. More importantly, there was no significant difference (ns) in cell directedness between the 5 Hz ramp EF and the DC EF, whereas the migration rate was better than that under the DC EF (P<0.001). Thus, 5 Hz ramp was the EF parameter with the best electrotaxis in this study. The results showed that low-frequency varying EFs could also induce cell directional migration, and the effects of EFs with different waveforms on the electrotaxis of the cells were significantly different. The 5 Hz ramp EF has the potential of application in both basic and clinical research.

参考文献/References

备注/Memo

备注/Memo:
(收稿日期:2021-04-16)国家自然科学基金资助项目(81927806,81772004)。△通信作者Email:lzpeng67@163.com
更新日期/Last Update: 2021-07-21