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Title:: A green synthesis method of fluorescent carbon quantum dots and its application

作者:: 陈鹏1; 2; 王超丽1; 王雪娇2; 吴红1; 杨锋2△; 1.空军军医大学药学系药物化学与分析教研室，西安 710000;2.空军军医大学第二附属医院胸外科，西安 710000

Author(s):: CHEN Peng1; 2; WANG Chaoli1; WANG Xuejiao2; WU Hong1; YANG Feng2; 1.Division of Pharmaceutical Chemistry and Analysis, Department of Pharmacy, Air Force Military Medical University, Xi＇an 710000, China;2.Department of Thoracic Surgery, the Second Affiliated Hospital of Air Force Military Medical University Xi＇an 71000

Keywords:: Fluorescent carbon quantum dots; Cell imaging; Bacteria imaging; Green synthesis; Fluorescence imaging

摘要:: 本研究探索一种可用于生物医学成像的绿色、高效的荧光碳量子点合成方法,以天然淀粉和水为原料，采用水热法合成碳量子点（carbon quantum dots,CQDs）。利用透射电镜和原子力显微镜表征CQDs形貌；紫外光谱、拉曼光谱等方法表征其光学性能，共聚焦显微镜检测其在金黄色葡萄球菌和肺癌A549细胞中的生物成像能力，采用CCK-8法和体内包埋实验验证CQDs的生物安全性。合成的CQDs粒径分布窄，平均粒径为2.3nm，具有良好的水分散性和较低的生物毒性。共聚焦荧光显微镜观察证实CQDs可对细胞和细菌进行成像。体内及体外安全性实验证实该材料具有良好的生物安全性。该CQDs的制备方法简单、成本低廉、生物相容性好，具有良好的生物成像功能，在生物医学等领域具有潜在的应用价值。

Abstract:: To explore a green and efficient synthesis method of fluorescent carbon quantum dots for biomedical imaging,we synthesized carbon quantum dots (CQDs) with natural starch and water by hydrothermal method. The morphology of CQDs was characterized by transmission electron microscopy and atomic force microscopy.The optical properties of CQDs were characterized by UV,Raman spectroscopy, etc. The imaging ability of CQDs in Staphylococcus aureus and A549 cells was detected by confocal microscopy. The biosafety of CQDs was verified by CCK-8 method and 〖WTBX〗in vivo〖WTBZ〗 embedding test.The particle size distribution of CQDs was narrow, andthe average size was 2.3 nm. It had good water dispersion and low toxicity. Confocal fluorescence microscopy confirmed that CQDs could image cells and bacteria. In vitro and in vivo experiments proved that the material had good biological safety.Thepreparation method of CQDS is simple with low cost, good biocompatibility, good biological imaging function, and has potential application value in biomedical and other fields.