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Title:: Construction of nanocomposite system for up-conversion mesoporous silica multi-functional liver cancer diagnosis and treatment

Author(s):: YANG Han; CHEN Xingmeng; GAO Junxiao; WU Xiaoli; CHANG Jin; Tianjin University, Tianjin 300072，China

Keywords:: Upconversion; Mesoporous silica; Indocyanine green; Hepatoma carcinoma; Liver cancer

摘要:: 本研究旨在设计一种用于肝癌诊疗用光激发载药上转换介孔二氧化硅纳米复合体系。首先应用油相法制备掺杂镱（Yb3+）、铒（Er3+）的上转换纳米颗粒（UCNP），并在颗粒表面包裹介孔二氧化硅（mSiO2），其次在UCNP@mSiO2中负载肝癌分子靶向治疗药物索拉菲尼（S）和光热荧光染料吲哚箐绿（ICG）。本研究利用可见光紫外光光谱，热成像等方法对纳米颗粒进行表征，并检测其载药性能和光热性能等。通过人肝癌细胞（HepG2）检测该纳米颗粒安全性、内吞效果及杀伤效果。实验结果表明该纳米颗粒具有较好的生物安全性，可在细胞内实现上转换荧光成像，并可有效杀伤肝癌细胞。本研究为该纳米颗粒在肝癌中的诊疗作用提供了有力支持。

Abstract:: To design a photoactivated drug-loaded upconversion mesoporous silica nanocomposite for the treatment of liver cancer. Firstly, the ytterbium (Yb3+), erbium (Er3+) doped upconversion nanoparticles (UCNP) were developed by oil phase method and covered with mesoporous silica (mSiO2) on the surface. Secondly, molecular targeted drug sorafenib (S) and photothermal fluorescent dye indocyanine green (ICG) were loaded in UCNP@mSiO2. In this study, visible light ultraviolet spectroscopy, thermal imaging and other methods were used to characterize drug-loading and photothermal properties of nanoparticles.Safety, endocytosis and cytotoxity effect of this nanoparticles were detected by human hepatoma cells (HepG2). These results indicate that this composite nanocarrier has good biosafety, can effectively kill tumor cells, and achieve up-conversion fluorescence imaging in tumor cells. Our study provides strong support for the diagnosis and treatment of liver cancer.