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Title:: Application of Hough Transform for the Processing of Data for PCR-reverse Dot Blotting

作者:: 王刚1; 李岭2△; 1.东北大学中荷生物医学与信息工程学院，沈阳 110819；2.四川大学生命科学学院遗传医学研究所，生物治疗国家重点实验室，成都 610041

Author(s):: WANG Gang1; LI Ling2; 1.Sino-Dutch Biomedical and Information Engineering School, Northeastern University, Shenyang 110819, China；2.State Key Laboratory for Biotherapy, Institute of Genetic Medicine, School of Life Science, Sichuan University, Chengdu 610041, China

Keywords:: Polymerase chain reaction-reverse dot blotting; Hough transform; Binary image; Median filter; Gene mutation screening; Mobile terminal

摘要:: 探讨霍夫变换（Hough transform）在聚合酶链反应-反向点杂交（polymerase chain reaction - reverse dot blotting，PCR-RDB）检测基因突变结果的自动化判读中的应用。以凯普HMM-2I医用核酸分子快速杂交仪检测基因突变为例，建立一种通过霍夫变换进行图像识别的方法，以快速判读PCR-RDB的检测结果，并提出一套基于移动终端的检测结果后处理方案。利用开发的软件，对113份地中海贫血常见突变以及86份人乳头瘤病毒分型的PCR-RDB检测结果进行了自动读取，并同人工判读结果进行了比较，二者的符合率均达100%。将霍夫变换应用于PCR-RDB检测图像的自动化判读符合实际需求，无需人工干预，可以降低肉眼判读的错误率并提高工作效率。

Abstract:: To explore the application of Hough transform for automated interpretation of the results of polymerase chain reaction-reverse dot blotting (PCR-RDB) for the detection of genetic mutations. The Hybribio HMM-2I Rapid Hybridizer for the detection of DNA mutations was used as an example. A method was established for fast interpretation of the PCR-RDB results by image recognition using Hough transform. In addition, a set of mobile-terminals-based results processing scheme were proposed. A software was developed for automatic interpretation of the PCR-RDB results for detecting common mutations in 113 thalassemia cases and subtypes of human papilloma virus in 83 cases. The results were compared with those of manual interpretation, with a consistency of 100%. The Hough transform can suit automated interpretation of the results of PCR-RDB assays. The method does not require human intervention, can reduce the error rate of visual interpretation, and improve the efficiency of testing.