[1]李奥琦,邓林红△,王翔△.细胞隧道纳米管结构组分对其形成和维持的影响*[J].生物医学工程研究,2022,02:114-121.
LI Aoqi,DENG Linhong,WANG Xiang.Effect of the components of tunneling nanotube on its formation and stability[J].Journal of Biomedical Engineering Research,2022,02:114-121.
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细胞隧道纳米管结构组分对其形成和维持的影响*(PDF)
《生物医学工程研究》[ISSN:1006-6977/CN:61-1281/TN]
- 期数:
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2022年02期
- 页码:
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114-121
- 栏目:
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- 出版日期:
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2022-06-25
文章信息/Info
- Title:
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Effect of the components of tunneling nanotube on its formation and stability
- 文章编号:
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1672-6278 (2022)02-0114-08
- 作者:
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李奥琦; 邓林红△; 王翔△
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常州大学生物医学工程与健康科学研究院,常州 213164
- Author(s):
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LI Aoqi; DENG Linhong; WANG Xiang
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Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, China
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- 关键词:
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细胞膜纳米管; 细胞间通讯; 微丝; 钙黏蛋白; 胆固醇; HEK293 细胞
- Keywords:
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Tunneling nanotube; Cell-cell communication; F-actin; Cadherin; Cholesterol; HEK293 cells
- 分类号:
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R318;R936
- DOI:
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10.19529/j.cnki.1672-6278.2022.02.03
- 文献标识码:
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A
- 摘要:
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由于细胞隧道纳米管(tunneling nanotube,TNT)生成的分子机制的多样性,目前尚未发现靶向TNT的低毒、广谱药物。本研究使用细胞松弛素D (cytochalasin D, cytoD)、乙二醇双(2-氨基乙基醚)四乙酸(EGTA)、甲基β环糊精(Mbc)分别破坏HEK293细胞间TNT结构的微丝、N型钙黏蛋白和胆固醇,来研究TNT结构组分对其形成和维持的影响。结果表明,破坏F-actin对TNT的生成频率的抑制作用最为明显,而对已存在的TNT的生存时间影响较小,钙黏蛋白是影响TNT的生存时间的关键分子,而胆固醇的水平对TNT的生成频率和生存时间的作用均有一定影响。本研究表明,通过干预TNT的结构组分控制TNT数量,有望成为筛选靶向 TNT 的药物的新方向。
- Abstract:
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Due to the diversity of the molecular mechanisms of formation, low-toxic, broad-spectrum drugs targeting tunneling nanotube(TNT)have not yet been developed. In order to study the influence of TNT structural components on its formation and maintenance, cytochalasin D (cytoD), ethylene glycol bis(2-aminoethyl ether) tetraacetic acid (EGTA), and methyl β cyclodextrin (Mbc) were used to destroy the microfilament,cadherin and cholesterol of the TNT structurein HEK293 cells. The results showed that destroyed F-actin had the most obvious inhibitory effect on the formation frequency of TNT, but had little effect on the lifetime of existing TNT. N-cadherin was a key molecule that affected the lifetime of TNT. The level of cholesterol had a certain effect on the formation frequency and life time of TNT. These results indicate that the control of TNT levels by interfering with its structural components is expected to provide a new direction for screening drugs targeting TNT.
备注/Memo
- 备注/Memo:
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(收稿日期:2022-01-11)国家自然科学基金资助项目(31972922);江苏省自然科学基金资助项目(BK20191452)。△通信作者Email:wangxiang@cczu.edu.cn;dlh@cczu.edu.cn
更新日期/Last Update:
2022-07-21