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Title:: Changes of trabecular meshwork structure and morphology induced by high intraocular pressure in rats

作者:: 王涛1; 2; 李林1; 2; 钱秀清1; 2; 张海霞1; 2; 刘志成1; 2△; 1.首都医科大学生物医学工程学院，北京 100069；2.首都医科大学临床生物力学应用基础研究北京市重点实验室，北京 100069

Author(s):: WANG Tao1; 2; LI Lin1; 2; QIAN Xiuqing1; 2; ZHANG Haixia1; 2; LIU Zhicheng1; 2; 1.School of Biomedical Engineering, Capital Medical University, Beijing 100069, China;2. Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, Beijing 100069

Keywords:: Rats; Trabecular meshwork; High intraocular pressure; Morphology; Density; Collagen fiber

摘要:: 小梁网的组织结构形态对青光眼的相关研究具有重要意义。本研究通过烙闭大鼠上巩膜静脉建立2周、3周、4周不同持续时间的高眼压动物模型，对眼球石蜡切片进行苯胺蓝染色和Masson染色，后续利用Image Pro Plus图像软件对小梁网密度和胶原纤维的变化情况进行分析。研究发现，小梁网密度在各个时间点上与对照组相比有统计学差异，且随时间变化明显；小梁网组织中蓝色胶原纤维面积在各个时间点上与对照组相比有统计学差异且与时间有相关性。研究表明，高眼压的持续作用会导致大鼠小梁网结构变得致密，胶原纤维增加且与持续时间相关。本研究探讨高眼压持续作用下小梁网的组织结构形态变化，为青光眼治疗的相关研究提供前期参考。

Abstract:: The structural morphology of trabecular meshwork is of great significance to the study of glaucoma.We established the rat models of high intraocular pressure with different durations of 2 weeks, 3 weeks and 4 weeks by cauterizing the upper scleral vein of rats. Aniline blue staining and Masson staining were performed on paraffin sections of the eyeball. Subsequently, Image Pro Plus was used to analyze the changes of trabecular meshwork density and collagen fiber. Compared with the control group, the trabecular meshwork density was statistically different at each time point and changed significantly over time. Compared with the control group, the area of blue collagen fiber in trabecular meshwork was statistically different at each time point and correlated with time.This study has shown that the continuous effect of high intraocular pressure can cause the structure of trabecular meshwork in rats to become dense and collagen fibers to increase, which is related to the duration. It investigates the structural and morphological changes of trabecular meshwork under the high intraocular pressure, providing a preliminary reference for related studies on glaucoma treatment.