|本期目录/Table of Contents|

[1]杨兴华△,张静,林静,等.脱钙牙本质基质-丝素蛋白支架对人牙周膜干细胞的成骨分化影响*[J].生物医学工程研究,2020,04:383-389.
 YANG Xinghua,ZHANG Jing,LIN Jing,et al.The effect of dentin-silk fibroin protein scaffold on osteogenic differentiation of human periodontal stem cells[J].Journal of Biomedical Engineering Research,2020,04:383-389.
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脱钙牙本质基质-丝素蛋白支架对人牙周膜干细胞的成骨分化影响*(PDF)

《生物医学工程研究》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2020年04期
页码:
383-389
栏目:
出版日期:
2020-12-25

文章信息/Info

Title:
The effect of dentin-silk fibroin protein scaffold on osteogenic differentiation of human periodontal stem cells
文章编号:
1672-3278(2020)04-0383-07
作者:
杨兴华12△张静3林静1李兵2王小娟1孙俊伟1
1.山东省烟台市业达医院口腔科, 烟台 264006;2.山东第一医科大学口腔医学院,泰安 271000; 3.滕州市中心人民医院口腔科,滕州 277500
Author(s):
YANG Xinghua12ZHANG Jing3LIN Jing1LI Bing2WANG Xiaojuan1SUN Junwei1
1.Department of Stomatology,Yantai Yeda Hospital,Yantai 264006,China;2.School of Stomatology,Shandong First Medical University,Taian 271000,China; 3.Department of Stomatology,Tengzhou Center Hospital,Tengzhou 277500,China
关键词:
丝素蛋白脱钙牙本质基质支架牙周膜干细胞骨修复
Keywords:
Silk fibroin Decalcified dentin matrixScaffoldHuman periodontal ligament stem cellsBone repair
分类号:
R318
DOI:
10.19529/j.cnki.1672-6278.2020.04.12
文献标识码:
A
摘要:
为研究脱钙牙本质基质(decalcified dentin matrix,DDM)与丝素蛋白 (silk fibroin,SF )复合多孔三维复合支架(DDM-SF)对体外培养的人牙周膜干细胞(human periodontal ligament stem cells,HPDLSC)成骨分化的影响。在-20℃冻干条件下,将6%SF、1%聚乙二醇 (polyethylene glycol,PEG)以体积比7:3(30%v/v)与DDM构建复合支架,红外光谱仪检测DDM-SF支架的特性波长,分别测定SF和DDM-SF的孔径和压缩强度,XRD测定DDM-SF的空间结构,BET检测复合材料的孔隙率。培养HPDLSC接种到支架上,CCK-8法检测细胞在支架上的增殖,扫描电镜观察细胞与支架的结合。采用研磨法提取支架细胞蛋白,行Westernblot检测。SF、DDM-SF分别埋入大鼠头部皮下后分期取出进行组织切片HE染色。DDMSF红外光谱特征吸收峰在3 442 cm-1、1 033 cm-1、3 500 cm-1谱峰;30%DDM-SF(v/v)孔径(106.6370±25.4454)μm、压缩强度(87.3333±13.6504) MPa。经检测DDM-SF的结构呈三维空间结晶结构,材料孔隙率98%。检测结果证实DDM-SF复合支架在促进成骨、细胞增殖、材料吸收速度方面均优于对照组。因此,经冷冻干燥条件下制作的多孔DDM-SF复合支架可促进HPDLSC的成骨分化和增殖。
Abstract:
To investigate the effect of porous scaffold (DDM-SF) fabricated by decalcified dentin matrix (DDM)and silk fibroin (SF) on osteogenic differentiation of human periodontal stem cells (HPDLSC) in vitro.The composite scaffolds were fabricated with 6% SF, 1% polyhexanol(PEG) and DDM at volume ratio 7:3(30%v/v) under -20℃ freeze-drying conditions. The characteristic wavelength of DDM-SF scaffold was detected by infrared spectrometer,the pore diameter and compression strength of SF and DDM-SF were measured respectively.The spatial structure of DDM-SF was determined by XRD and the porosity of the scaffold was determined by BET.HPDLSC were cultured and inoculated on the scaffold. The proliferation of the cells on the scaffold was detected by cck-8 method, and the combination of the cells on the scaffold was observed by SEM. To extract protein of cells on the scaffold by grinding method for Westernblot.SF and DDM-SF were respectively embedded under the skin of the rat head and then were taken out by stages for HE staining in tissue sections.The absorption spectrum peak of DDM-SF were 3 442 cm-1,1 033 cm-1,3500 cm-1. 30%DDM-SF diameter was (106.6370±25.4454) μm, compression strength was (87.3333±13.6504) MPa. XRD results showed that the DDM-SF scaffold was three - dimensional crystalline structure.BET results showed the porosity of the material was 98%.Cck-8 results showed mild inhibition to cells was at the initial stage of detection.Western blot and HE results confirmed that DDM-SF scaffold was better than the control group in promoting osteogenesis, cell proliferation and material absorption rate.The porous DDM-SF composite scaffold prepared under freeze-dried conditions can promote the osteogenic differentiation and proliferation of HPDLSC.

参考文献/References

备注/Memo

备注/Memo:
(收稿日期:2020-02-21)山东省高等学校科技计划项目(J13LL01)。△通信作者Email:yang720913@sina.com
更新日期/Last Update: 2021-02-07